Ethanolic Extract Gel Of Planchonia Valida Leaves As An Antibacterial Agent Against Yersiniosis Infection

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Vol. 22 No. 1 (2025): Jurnal Kesehatan Lingkungan Volume 22 No. 1, Januari 2025
Published: Jan 27, 2025
Keywords:
Planchonia valida,
Yersinia enterocolitica,
Antimicrobial Activity,
Ethanol Extract,
Inhibition Zone

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Purwita Sari
Department of Biology Education (Tadris Biologi), State Islamic Institute of Palangka Raya
Noor Hujjatusnaini
Department of Biology Education (Tadris Biologi), State Islamic Institute of Palangka Raya
Ridha Nirmalasari
Department of Biology Education (Tadris Biologi), State Islamic Institute of Palangka Raya

Abstract

Planchonia valida (puting plant) contains ethanol, which is beneficial for treating skin infections and digestive problems, and is capable of inhibiting the growth of gram-negative bacteria such as Yersinia enterocolitica. This study aims to evaluate the adaptability of P. valida extract to Y. enterocolitica in vitro. This experimental research was statistically analyzed using Statistical Product and Service Solutions (SPSS). The adaptability of Y. enterocolitica was observed on nutrient agar (NA) medium in petri dishes treated with P. valida extract at concentrations of 30%, 40%, 50%, 60%, 70%, and 80%, with aquadest as the negative control and chloramphenicol as the positive control. Observations were conducted over three incubation periods (24, 48, and 72 hours) to monitor bacterial growth rate and adaptation. ANOVA results showed p-values at all incubation periods were below the significance level (α = 0.05), indicating significant differences in bacterial adaptation across treatments. The inhibition zone test revealed that the 40% extract concentration was as effective as higher concentrations (50%, 60%, 70%, and 80%). Although the 80% concentration exhibited the largest inhibition zone, its bactericidal effect was not statistically different from the lower concentrations. These results demonstrate the potential of Planchonia valida leaf extract as an effective antimicrobial agent and support further research into its development for the treatment or prevention of Y. enterocolitica infections.

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References

WHO. Dengue and severe dengue [Internet]. Who. 2023. p. 1–13. Available from: http://www.who.int/mediacentre/factsheets/fs117/en/%0Ahttps://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue%0Ahttps://www.who.int/health-topics/dengue-and-severe-dengue#tab=tab_1

Kementerian Kesehatan Republik Indonesia RI. Strategi Nasional Penanggulangan Dengue 2021-2025 [Internet]. Kementerian Kesehatan RI. 2019. 1 p. Available from: https://www.kemkes.go.id/article/view/19093000001/penyakit-jantung-penyebab-kematian-terbanyak-ke-2-di-indonesia.html.Kemenkes

Andriawan FR, Kardin L, Rustam HN M. Hubungan Antara Status Gizi dengan Derajat Infeksi Dengue Pada Pasien Demam Berdarah Dengue. Nurs Care Heal Technol J. 2022;2(1):8–15.

Kemenkes RI. Kasus DBD Meningkat, Kemenkes Galakkan Gerakan 1 Rumah 1 Jumantik (G1R1J). Kemenkes RI. 2022.

Kementrian Kesehatan RI. Membuka Lembaran Baru. LAPORAN TAHUNAN 2022. Demam Berdarah Dengue [Internet]. Germas. 2022. Available from: http://p2p.kemkes.go.id/wp-content/uploads/2023/06/FINAL_6072023_Layout_DBD-1.pdf

Dikshith TSS. Handbook of chemicals and safety [Internet]. Handbook of Chemicals and Safety. 2016. p. 1–474. Available from: https://agriculture.basf.com/global/en/business-areas/public-health/products/abate.html

Triyana R, Putri TA, Primawati I, Susanti M, Adelin P, Salmi S. Efektivitas Larvasida Infusa Bunga Lawang (Illicium Verum) Terhadap Mortalitas Larva Aedes Aegypti Instar III. Malahayati Nurs J. 2022;4(11):3130–54.

Rochmat A, Adiati MF, Bahiyah Z. Pengembangan Biolarvasida Jentik Nyamuk Aedes aegypti Berbahan Aktif Ekstrak Beluntas (Pluchea indica Less.). Reaktor. 2017;16(3):103.

Setiawan A, Setyo Yudo T. Teknologi Produksi Tanaman Rempah dan Aroma. Malang: UB Press; 2023. 147 p.

USDA (United States Department of Agriculture). Spices and Herbs [Internet]. 2018. Available from: https://www.fs.usda.gov/wildflowers/ethnobotany/food/spices.shtml#:~:text=Spices and herbs are defined,flowers%2C fruits%2C and seeds.

Listianda AS. Berbagai Rempah-rempahan dan Manfaatnya [Internet]. Elementa Media; 2021. 63 p. Available from: https://www.google.co.id/books/edition/Berbagai_Rempah_rempahan_dan_Manfaatnya/ue93EAAAQBAJ?hl=en&gbpv=1

Veer V, Gopalakrishnan R. Herbal insecticides, repellents and biomedicines: Effectiveness and commercialization. Herbal Insecticides, Repellents and Biomedicines: Effectiveness and Commercialization. 2016. 1–258 p.

Sadino A, Nauri D., Masturoji DE., Apriani R. Larvicide Activity and Anti-Mosquito Activity of Several Plants in Indonesia Against Aedes Aegypti: Review Articles. 2023;

Yatuu US, Jusuf H, Lalu NAS. PENGARUH PERASAN DAUN SERAI DAPUR (Cymbopogon citratus) TERHADAP KEMATIAN LARVA Aedes aegypti. Jambura J Heal Sci Res. 2020;2(1):32–42.

Mulyani S. Lemongrass oil granules as Aedes aegypti larvacide. Tradit Med J. 2014;19(September):138–41.

Koraag ME. Lethal Time Ekstrak Bunga Kecombrang (Etlingera elatior) Terhadap Larva Aedes aegypti. Semin Nas Biol [Internet]. 2020;300–9. Available from: http://103.76.50.195/semnasbio/article/view/15307

Zikrillah A, Kristinawati E, Fihiruddin. Uji efektifitas filtrat rimpang lengkuas putih ( Alpinia galangal Swartz ) sebagai lsrvasida Aedes sp. Anal Med Bio Sains. 2017;4(2):99–103.

Boesri H, Heriyanto B, Handayani SW, Suwaryono T. UJI TOKSISITAS BEBERAPA EKSTRAK TANAMAN TERHADAP LARVA AEDES AEgYPTI VEKTOR DEMAM BERDARAH DENGUE. Vektora J Vektor dan Reserv Penyakit. 2015;7(1).

Syukrillah F. Komposisi kimia dan aktivitas larvasida Aedes aegypti minyak kayu putih dari berbagai sentra produksi di Indonesia. 2014;

Nurhaifah D, Sukesi TW. Efektivitas Air Perasan Kulit Jeruk Manis sebagai Larvasida Nyamuk Aedes aegypti. Kesmas Natl Public Heal J. 2015;9(3):207.

Kartika FD, Isti’anah S. EFEK LARVISIDA EKSTRAK ETANOL DAUN KEMANGI (Ocimum sanctum Linn) TERHADAP LARVA INSTAR III Aedes aegypti. J Kedokt dan Kesehat Indones. 2014;6(1):38–46.

Asyuri FA, Ringoringo VS. Uji AKtivitas Larvasida Ekstrak Etanol 70% Daun Kerehau (Callicarpa longifolia Lam.) Terhadap Larva Nayamuk Aedes aegypti Instar III. Indones Nat Res Pharm J. 2018;3(1):142–9.

Riki M, Praristiya S, Udiyani R, Dewi TS. FORMULASI GRANUL LARVASIDA EKSTRAK DAUN LEMON (Citrus Limon)SEBAGAI LARVASIDA ALAMI YANG RAMAH LINGKUNGAN. 2019;1–0.

Aljameeli M. Larvicidal effects of some essential oils against Aedes aegypti (L.), the vector of dengue fever in Saudi Arabia. Saudi J Biol Sci. 2023;30(2).

Chaves R do SB, Martins RL, Rodrigues ABL, Rabelo É de M, Farias ALF, Brandão LB, et al. Evaluation of larvicidal potential against larvae of Aedes aegypti (Linnaeus, 1762) and of the antimicrobial activity of essential oil obtained from the leaves of Origanum majorana L. PLoS One. 2020;15(7 July).

Laojun S, Damapong P, Damapong P, Wassanasompong W, Suwandittakul N, Kamoltham T, et al. Efficacy of commercial botanical pure essential oils of garlic (Allium sativum) and anise (pimpinella anisum) against larvae of the mosquito aedes aegypti. J Appl Biol Biotechnol. 2020;8(6):88–92.

WHO/CDS/WHOPES. Guidelines for laboratory and field testing of mosquito larvicides. World Heal Organ [Internet]. 2005;10:1–41. Available from: http://whqlibdoc.who.int/hq/2005/WHO_CDS_WHOPES_GCDPP_2005.13.pdf?ua=1

Kumara CJ, Nurhayani, Bestari RS, Dewi LM. Efektivitas Flavonoid , Tanin , Saponin dan Alkaloid terhadap Mortalitas Larva Aedes aegypty. Iniversity Res Colloqium. 2021;(13):106–18.

Roy R. Percentages and parts per million (ppm) [Internet]. Environment: treading lightly on the Earth. 2019. Available from: https://www.open.edu/openlearn/nature-environment/environmental-studies/environment-treading-lightly-on-the-earth/content-section-1.4.1#:~:text=Parts per million (ppm) is,useful for very small proportions.

Manh HD, Hue DT, Hieu NTT, Tuyen DTT, Tuyet OT. The mosquito larvicidal activity of essential oils from cymbopogon and eucalyptus species in vietnam. Insects. 2020;11(2).

Komisi Pestisida Departemen Pertanian. Metode Standar Pengujian Efikasi Pestisida. Jakarta: Departemen Pertanian; 2012.

Magalhaes Xavier KC, Dias do Nascimento Santos DK, Dantas da Cruz RC, Macena da Silva LQ, Silva Araújo AM, Santos PMD, et al. Phytochemical, cytotoxic, and insecticidal effects of crude extracts of the alga Alsidium triquetrum (SGGmelin) Trevisan on Aedes aegypti. 2025;63.

Galavíz-Parada JD, Vega-Villasante F, Cupul-Magaña FG, Navarrete-Heredia JL, Ruiz González LE, Vargas-Ceballos MA, et al. Chemical and biological control on Aedes aegypti larvae in the northern coastal region of Jalisco, Mexico. 2016;68(2):111–24.

Moura L, de Nadai BL, Corbi JJ. What does not kill it does not always make it stronger: High temperatures in pyriproxyfen treatments produce Aedes aegypti adults with reduced longevity and smaller females: Effects of pyriproxyfen on life history traits of Aedes aegypti. 2020;23(2):529–35.

Manzano P, García OB, Malusín J, Villamar J, Quijano M, Viteri R, et al. Larvicidal activity of ethanolic extract of azadirachta indica against aedes aegypti larvae. 2020;73(3):9315–20.

Prada-Ardila RA, Jiménez-Umbarila SE, Dussán-Cárdenas NM, Corradine-Mora DT. Control of larvae Aedes aegypti with extracts of Allium sativum and Annona muricata as larvicides. 2021;23(3).

Amorim QS, da Rocha Bauzer LGS, Braga IA, Lima JBP. Evaluation of the persistence of three larvicides used to control aedes aegypti in arapiraca, northeastern Brazil. 2020;35(3):192–9.

Promsiri S, Naksathit A, Kruatrachue M, Thavara U. Evaluations of larvicidal activity of medicinal plant extracts to Aedes aegypti (Diptera: Culicidae) and other effects on a non target fish. 2006;13(3):179–88.