Effectiveness of Boiled Sapodilla Leaves (Manilkara zapota) in Reducing the Number of Bacteria and Escherichia coli Germs on Eating Utensils

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Vol. 21 No. 2 (2024): Jurnal Kesehatan Lingkungan Volume 21 No. 2, Juli 2024
Published: Jul 1, 2024
Keywords:
Sapodilla leaf boiled water,
Escherichia coli,
Germ count,
Sanitizing

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Laili Agustina
Poltekkes Kemenkes Banjarmasin Jurusan Kesehatan Lingkungan
Rahmawati Rahmawati
Poltekkes Kemenkes Banjarmasin Jurusan Kesehatan Lingkungan
Erminawati Erminawati
Poltekkes Kemenkes Banjarmasin Jurusan Kesehatan Lingkungan
Abdul Haris
Poltekkes Kemenkes Banjarmasin Jurusan Kesehatan Lingkungan

Abstract

Several studies have shown that the high number of germs in cutlery is due to the inability to carry out the sanitizing process. The sanitizing process can be done physically and chemically, using natural ingredients that contain active compounds such as flavonoids, tannins, and saponins, which function as antibacterials, such as sapodilla leaves. Sapodilla leaf boiled water can be a simple disinfectant for society to improve the quality of cutlery hygiene. The study aimed to determine the concentration of sapodilla leaf boiled water, which effectively reduces E. coli and the number of germs. The statistical test used was Kruskal Wallis, followed by a nonparametric post hoc test. A total of 24 swab samples were used with eight treatments and three repetitions. The results showed that the examination for the presence of E. coli on cutlery was negative. Examination of the germ count showed that the highest germ rate was in the control and the lowest was 10%. Statistical test results showed that a concentration of 10% is effective in reducing the germ number. This study concludes that E. coli in the samples were all negative, and the effective concentration in reducing the number of germs on cutlery is 10%, reducing from 60,768.11 colonies/cm2 to 12 colonies/cm2. Suggestions for future researchers are to conduct trials using other extraction methods.

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References

BPOM. Pedoman Kriteria Cemaran pada Pangan Siap Saji dan Pangan Industri Rumah Tangga [Internet]. 2012. Available from: https://standarpangan.pom.go.id/dokumen/pedoman/Buku_Pedoman_PJAS_tentang_Cemaran.pdf

BPOM. Laporan tahunan 2021 Badan Pengawas Obat dan Makanan. WwwPomGoId 2022;

Marisdayana R, Harahap PS, Yosefin H. Teknik Pencucian Alat Makan, Personal Hygiene Terhadap Kontaminasi Bakteri Pada Alat Makan. J Endur 2017;2(3):376.

Tumelap HJ. Tikala Manado. Kondisi Bakteriol Peralat Makan Di Rumh Makan Jombang Tikala Manad 2011;1(1):20–7.

PERMENKES No. 1096/MENKES/PER/VI/2011 Tentang Hygiene Sanitasi Jasaboga. Angew Chemie Int Ed 6(11), 951–952 1967;2008(Mi):5–24.

Budon AS. Studi Kualitas Bakteriologis Air Pencucian Dan peralatan Makan Di Kantin UIN Makasar. آب و خاک 2013;(May):106.

Telew M, Joseph WBS, Pinontoan O, Masyarakat FK, Ratulangi US. Gambaran Angka Kuman Dan Keberadaan Escherichia Coli Pada Peralatan Makan Rumah Makan Di Kelurahan Mahakeret Barat Dan Mahakeret Timur Kecamatan Wenang Kota Manado. Kesmas 2019;7(5):5–8.

DepKes. Standar Kegiatan Usaha dan Produk Pada penyelenggaraan Perizinan Berusaha Berbasis Risiko Sektor Kesehatan. Permenkes No 14 2021;455.

Amaliyah N. No Title. Yogyakarta: Deepublish; 2017.

Amaliyah N. Penyehatan Makanan Minuman. Yogyakarta: Deepublish; 2017.

Islamiar AN, . N, Rahayu U. Efektivitas Perasan Daun Jeruk Purut Terhadap Angka Kuman Pada Peralatan Makan Tahun 2017. Gema Lingkung Kesehat 2017;15(3):50–5.

Prihardini, Wiyono AS. Pengembangan Dan Uji Antibakteri Ekstrak Daun Sawo Manila ( Manilkara Zapota ) Sebagai Lotio Terhadap Staphyllococcus aures The Development And Antibacteria Test Of Manila Sapodilla Leaf ( Manilkara Zapota ) AS A LOTIO TO Staphyllococcus aures. J Wiyata, 2019;2(1):87–92.

Puspaningtyas desty ervira. The Miracles of Fruits. PT AgroMedia Pustaka; 2013.

Steenis CGG. Van. Flora. Kebon Raya Indonesia; 1953.

Yunika N. Konsentrasi Hambat Minimum Ekstrak Daun Sawo (Achras Zapota L.) Terhadap Pertumbuhan Staphylococcus Aureus Secara In Vitro. Bioscience 2017;1(1):53.

Mustary M, Djide MN, Mahmud I, Hasyim N. Uji Daya Hambat dan Analisis KLT-Bioautografi Perasan Buah Sawo Manila (Achras zapota Linn) Terhadap Bakteri Uji Salmonella Thyposa. Angew Chemie Int Ed 6(11), 951–952 [Internet] 2018;3(1):10–27. Available from: https://medium.com/@arifwicaksanaa/pengertian-use-case-a7e576e1b6bf

Rahayu WP, Nurjanah S, Komalasari E. Escherichia coli: Patogenitas,Analisis, dan Kajian Risiko. J Chem Inf Model 2018;1(5):1–151.

Mufti N, Bahar E, Arisanti D. Uji Daya Hambat Ekstrak Daun Sawo terhadap Bakteri Escherichia coli secara In Vitro. J Kesehat Andalas 2017;6(2):289.

M M. Brock Biology of Microorganisme. london: PrenticeHall; 2005.

Islam MR, Parvin MS, Banu MR, Jahan N, Das N, Islam ME. Antibacterial and phytochemical screening of ethanol extracts of Manilkara zapota leaves and bark. Int J Pharma Sci [Internet] 2013;3(6):394–7. Available from: http://ijps.aizeonpublishers.net/content/2013/6/ijps394-397.pdf

Pratiwi N, Retnosari R, Prabaningtyas S. Preliminary Study on Antibacterial Activity of Sawo Kecik (Manilkara kauki (L.) Dubard) Roots Extract. J Biodjati 2021;6(1):146–52.

Dewi Anggraini IAK, Trisna Darmayanti LP, Sugitha IM. PENGARUH LAMA PEREBUSAN PADA PEMBUATAN MINUMAN HERBAL DAUN SAWO (Manilkara zapota) TERHADAP KARAKTERISTIK DAN DAYA HAMBAT PERTUMBUHAN Escherichia coli. J Ilmu dan Teknol Pangan 2020;9(3):272.

Indah SJ. Uji alternatif kruskal wallis & post hoc. 2023;

Purwanto. Statistika untuk Penelitian. Yogyakarta: Pustaka belajar; 2001.

Hasyim, Muh F, Patandung G, Irfiana. Uji Aktivitas Antibakteri Infusa Daun Sawo (Manilkara zapota L) Terhadap Escherichia coli. Pap Knowl Towar a Media Hist Doc 2014;16–9.